biotin labelled crna (Thermo Fisher)

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Thermo Fisher biotin labelled crna
Biotin Labelled Crna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotin+labelled+crna/D(%2B)-BIOTIN/pm39702367-112-15-33
Average 99 stars, based on 1 article reviews

biotin labelled crna - by Bioz Stars, 2026-09

99/100 stars

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Expressing:

Article Title: Prenatal transcript levels and metabolomics analyses reveal metabolic changes associated with intrauterine growth restriction and sex
Article Snippet: Affymetrix microarrays (Affymetrix Snowball, Geo Platform GPL16569) with 47 880 probe-sets were used. .. 500 ng RNA of each of the 24 samples was transcribed first into cDNA and then into biotin-labelled cRNA and hybridized onto the arrays using the Affymetrix WT plus Expression Kit and the Genechip WT terminal labelling and hybridization kit according to the manufacturer's instructions (Affymetrix, Santa Clara, CA, USA). .. Hybridization, washing and scanning of the arrays were performed on Affymetrix hybridization ovens, fluidics stations and scanners and the Affymetrix GCOS1.1.1 software was used for quality control.

Hybridization:

Reverse Transcription:

Article Title: The RRP9-JUN axis promotes breast cancer progression via the AKT signalling pathway
Article Snippet: Total RNA was extracted from BT549 cells transfected with shCtrl or shRRP9 using TRIzol reagent according to the manufacturer’s instructions, after which the RNA was analysed with a NanoDrop 2000 (Thermo Fisher Scientific, MA, USA). .. Subsequently, the RNA was reverse-transcribed into cDNA, labelled with 3’ IVT Biotin Label to generate biotin-labelled cRNA, and then hybridized using the GeneChip Prime-view Human Gene Expression Array according to the manufacturer’s instructions (Affymetrix, Santa Clara, CA). ..

Gene Expression:

Article Title: The RRP9-JUN axis promotes breast cancer progression via the AKT signalling pathway.
Article Snippet: Total RNA was extracted from BT549 cells transfected with shCtrl or shRRP9 using TRIzol reagent according to the manufacturer’s instructions, after which the RNA was analysed with a NanoDrop 2000 (Thermo Fisher Scientific, MA, USA). .. Subsequently, the RNA was reversetranscribed into cDNA, labelled with 3’ IVT Biotin Label to generate biotin-labelled cRNA, and then hybridized using the GeneChip Prime-view Human Gene Expression Array according to the manufacturer’s instructions (Affymetrix, Santa Clara, CA). ..

In Vitro:

Article Title: Neuronal DNA damage response-associated dysregulation of signalling pathways and cholesterol metabolism at the earliest stages of Alzheimer-type pathology.
Article Snippet: .. At the end of this round, after synthesis of double-stranded cDNA, biotin-labelled cRNA was prepared using the Affymetrix Gene Chip (Affymetrix, Santa Clara, CA, USA) in vitro transcription labelling kit. .. Following clean-up of the biotin-labelled cRNA the material was assayed (Agilent Bioanalyser 2100) to ensure sufficient RNA of appropriate quality had been prepared.

Review